Our Resources
- The CRG.BMD consortium is housed within the 30,000 sq ft NITTE University Centre for Science, Education and Research (NUCSER)
- All the equipment and facilities needed for our workflow are available to us at NUCSER
- CRG.BMD will have access to patients from the Justice K S Hegde Charitable Hospital, the teaching hospital associated with the K. S. Hegde Medical Academy, which is a constituent college of the NITTE (Deemed to be University) family of institutions.
- The K.S. Hegde Charitable Hospital is:
- A 1000 bed multi-specialty tertiary care hospital
- Offers patients expertise in 12 broad specialties, 13 super specialties and 4 clinical support services
- Offers state-of-the-art facilities catering to all branches of medicine and surgery
- Serves as the tertiary care hospital to 21 primary and 4 secondary care hospitals spread across three districts of Karnataka and Kerala
- In addition we will have access to:
- The state of the art Eye hospital
- Blood Bank (24/7)
- Department of Hematology/Oncology
- Department of Community Medicine
- Department of Public Health
- Patient samples and the accompanying clinical information will also be procured from our partners and collaborators
Lab Equipments
The following Lab equipment, among others, is available for CRG.BMD use in a state of the art laboratory:
- Nanopore DNA Sequencer
- Real Time PCR machine (Bio Rad and ABI systems)
- Droplet Digital PCR (Qx200, Bio Rad)
- HPLC Analytical system (Waters)
- Liquid Nitrogen Storage Tanks
- -80oC/-200C Freezers
- Centrifuges (Eppendorf), including Ultracentrifuges (Optima XPN 100, Beckman Coulter)
- Laminar Flow hoods (Imported and Indigenous)
- Biosafety Cabinets (Level II, Thermo)
- CO2 incubators (Thermo)
- Inverted Microscopes (Olympus)
- PCR Machines (BioRad)
- Gel Doc System (BioRad)
- Inject Man (Eppendorf)
- Fluorescent Microscopes (DM2500, Leica, BX51, Olympus)
- Zoe Fluorescent Cell Imager (Bio Rad)
- Phase contrast Microscope (Radical Instruments)
- Real Time PCR machine (Bio Rad and ABI systems)
- Droplet Digital PCR (Qx200, Bio Rad)
- HPLC Analytical system (Waters)
- Liquid Nitrogen Storage Tanks
- Centrifuges (Eppendorf), including Ultracentrifuges (Optima XPN 100, Beckman Coulter)
- Laminar Flow hoods (Imported and Indigenous)
- Biosafety Cabinets (Level II, Thermo)
- CO2 incubators (Thermo)
- Inverted Microscopes (Olympus)
- Fluorescent Microscopes (DM2500, Leica, BX51, Olympus)
- Zoe Fluorescent Cell Imager (Bio Rad)
- Phase contrast Microscope (Radical Instruments)
We also have access to a Beckman Coulter FACS machine.
Zebra fish facility
The Zebrafish facility is housed in NUCSER and is designed to model rare genetic human
diseases in a zebrafish model.
- The zebrafish facility comprises a completely automated, state- of- the- art re-circulatory system imported from Tecniplast, Italy. The unit involves a multilinking system capable of housing zebrafish in different sizes of tanks ranging from 1.1 litres to 3.5 litres capacity with an automated water treatment unit, that includes a fine mechanical filter, carbon filtration and high UV disinfection.
- The current facility has two multilinking racks with 6 tiers each capable of accommodating 120 rearing tanks
- The facility presently houses wildtype as well as a variety of transgenic and mutant zebrafish lines.
CRG.BMD Biorepository
- The CRG>BMD bio-repository has been planned as a best practice operative. Informed consent for collection of all bio-specimens will be obtained (see Documents and MoU) according the the Study Protocol previously approved by the IRB of NITTE DU, or the Institution/Hospital where samples will be collected
- With bio-specimen collection ongoing for rare genetic and bone
marrow failure
syndromes, the following patient samples will be bar-coded and
archived for
analysis:
- Blood
- Genomic DNA
- Total RNA
- All barcoded specimens will be stored in a specific traceable location in a specifically designated -80 deg C freezer
- The Privacy of each patient will be strictly maintained
- Sample specimens will be shared with collaborators responsible for depositing the samples in the CRG.BMD Bio-repository, upon written request
- Samples requested by other Researchers will need approval from CRG.BMD as well as Collaborators responsible for depositing the sample in the CRG.BMD Bio-Repository
Bringing together clinicians and researchers under one platform to improve clinical therapies and outcomes for the patients.
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