CRGBMD-RESOURCES

Our Resources

Resources Lab-Equipment Zebrafish-Facility Bio-Repository

The CRG.BMD consortium is housed within the 30,000 sq ft NITTE University Centre for Science, Education and Research (NUCSER)
All the equipment and facilities needed for our workflow are available to us at NUCSER
CRG.BMD will have access to patients from the Justice K S Hegde Charitable Hospital, the teaching hospital associated with the K. S. Hegde Medical Academy, which is a constituent college of the NITTE (Deemed to be University) family of institutions.
The K.S. Hegde Charitable Hospital is:
- A 1000 bed multi-specialty tertiary care hospital
- Offers patients expertise in 12 broad specialties, 13 super specialties and 4 clinical support services
- Offers state-of-the-art facilities catering to all branches of medicine and surgery
- Serves as the tertiary care hospital to 21 primary and 4 secondary care hospitals spread across three districts of Karnataka and Kerala
- In addition we will have access to:
  - The state of the art Eye hospital
  - Blood Bank (24/7)
  - Department of Hematology/Oncology
  - Department of Community Medicine
  - Department of Public Health
Patient samples and the accompanying clinical information will also be procured from our partners and collaborators

The following Lab equipment, among others, is available for CRG.BMD use in a state of the art laboratory:

Nanopore DNA Sequencer
Real Time PCR machine (Bio Rad and ABI systems)
Droplet Digital PCR (Qx200, Bio Rad)
HPLC Analytical system (Waters)
Liquid Nitrogen Storage Tanks
-80oC/-200C Freezers
Centrifuges (Eppendorf), including Ultracentrifuges (Optima XPN 100, Beckman Coulter)
Laminar Flow hoods (Imported and Indigenous)
Biosafety Cabinets (Level II, Thermo)
CO2 incubators (Thermo)
Inverted Microscopes (Olympus)
PCR Machines (BioRad)
Gel Doc System (BioRad)
Inject Man (Eppendorf)
Fluorescent Microscopes (DM2500, Leica, BX51, Olympus)
Zoe Fluorescent Cell Imager (Bio Rad)
Phase contrast Microscope (Radical Instruments)
Real Time PCR machine (Bio Rad and ABI systems)
Droplet Digital PCR (Qx200, Bio Rad)
HPLC Analytical system (Waters)
Liquid Nitrogen Storage Tanks
Centrifuges (Eppendorf), including Ultracentrifuges (Optima XPN 100, Beckman Coulter)
Laminar Flow hoods (Imported and Indigenous)
Biosafety Cabinets (Level II, Thermo)
CO2 incubators (Thermo)
Inverted Microscopes (Olympus)
Fluorescent Microscopes (DM2500, Leica, BX51, Olympus)
Zoe Fluorescent Cell Imager (Bio Rad)
Phase contrast Microscope (Radical Instruments)

We also have access to a Beckman Coulter FACS machine.

The Zebrafish facility is housed in NUCSER and is designed to model rare genetic human diseases in a zebrafish model.

The zebrafish facility comprises a completely automated, state- of- the- art re-circulatory system imported from Tecniplast, Italy. The unit involves a multilinking system capable of housing zebrafish in different sizes of tanks ranging from 1.1 litres to 3.5 litres capacity with an automated water treatment unit, that includes a fine mechanical filter, carbon filtration and high UV disinfection.
The current facility has two multilinking racks with 6 tiers each capable of accommodating 120 rearing tanks
The facility presently houses wildtype as well as a variety of transgenic and mutant zebrafish lines.

The CRG>BMD bio-repository has been planned as a best practice operative. Informed consent for collection of all bio-specimens will be obtained (see Documents and MoU) according the the Study Protocol previously approved by the IRB of NITTE DU, or the Institution/Hospital where samples will be collected
With bio-specimen collection ongoing for rare genetic and bone marrow failure syndromes, the following patient samples will be bar-coded and archived for analysis:
- Blood
- Genomic DNA
- Total RNA
All barcoded specimens will be stored in a specific traceable location in a specifically designated -80 deg C freezer
The Privacy of each patient will be strictly maintained
Sample specimens will be shared with collaborators responsible for depositing the samples in the CRG.BMD Bio-repository, upon written request
Samples requested by other Researchers will need approval from CRG.BMD as well as Collaborators responsible for depositing the sample in the CRG.BMD Bio-Repository